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Invitrogen™ Human IL-17AF (heterodimer) Uncoated ELISA Kit

Catalog No. 5017245
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10 x 96 Tests
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5017245 10 x 96 Tests
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Catalog No. 5017245 Supplier Invitrogen™ Supplier No. 88711788

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Description

ELISA

The Human IL-17AF (heterodimer) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17AF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Specifications

Accession Number Q16552,Q96PD4
Assay Range 30 to 4,000 pg/mL
Assay Sensitivity 30 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 112744, 3605
Gene Symbol IL-6
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Assay Diluent: 5X concentrated, Wash Buffer: 10X concentrated (100 mL of 10X per plate), Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Stop Solution: 5 mLs of 1X solution per plate, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym Interleukin-IL-17 AF, IL17AF
Target IL-17 AF
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
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