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Invitrogen™ Human IgG3 Uncoated ELISA Kit with Plates

Catalog No. 501129257
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2 x 96 Tests
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501129257 2 x 96 Tests
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Catalog No. 501129257 Supplier Invitrogen™ Supplier No. 885058022

Description

ELISA

The Human Immunoglobulin G3 (IgG3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgG3. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human IgG3, also known as human immunoglobulin G3, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG3 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG3 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG3 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.

Specifications

Assay Range 4.7 to 300 ng/mL
Assay Sensitivity 4.7 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Human IgG3 protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 2 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL
Storage Requirements 2°C to 8°C
Synonym Immunoglobulin G3
Target Human IgG3
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
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