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  5. Invitrogen™ Human IgG1 ELISA Kit

Invitrogen™ Human IgG1 ELISA Kit

Catalog No. BMS2092FIVE
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2 x 96 Tests
5 x 96 Tests
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BMS2092FIVE 5 x 96 Tests
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Description

ELISA

The Human Immunoglobulin G1 (Hu IgG1) ELISA quantitates Hu IgG1 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG1. Principle of the method The Human IgG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgG1, also known as human immunoglobulin G1, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the most abundant subclass of IgG in the blood and plays a key role in defense against pathogens. IgG1 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG1 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG1 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.

Specifications

Assay Range 15.6-1000 ng/mL
Assay Sensitivity 0.32 ng/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human IgG1
HRP-Conjugate anti-human IgG monoclonal antibody
human IgG1 Standard lyophilized, 2 000 ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias IGHG1; immunoglobulin heavy constant gamma 1 (G1m marker)IGHG1; immunoglobulin heavy constant gamma 1 (G1m marker);
Interassay CV 3.8%
Intraassay CV 2.7%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL
Storage Requirements 2-8°C
Target Human IgG1
Test Time 1 hr 20 min
Total Assay Time 1 hr 30 min
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For Research Use Only