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Gibco™ Human GDNF, Animal-Free Recombinant Protein, PeproTech®

Catalog No. p-7245759
Encompass
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1 mg
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Catalog No. Quantity
AF45010100 100 μg
AF4501010UG 10 μg
AF450101MG 1 mg
AF45010500 500 μg
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Catalog No. AF45010100 Supplier Gibco™ Supplier No. LSAF45010100UG
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Recombinant Protein

The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Specifications

Accession Number P39905
For Use With (Application) Functional Assay
Formulation protein with no preservative
Gene ID (Entrez) 2668
Molecular Weight (g/mol) 30.4 kDa
Name Human GDNF, Animal-Free
Quantity 100 μg
Source E. coli
Regulatory Status RUO
Endotoxin Concentration <0.1 EU/ μg
Gene Alias AI385739; Astrocyte-derived trophic factor; ATF; ATF1; ATF2; Gdnf; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; Glial cell line derived neutrophic factor; glial cell line-derived neurotrophic factor; gndf; HFB1-GDNF; hGDNF; H-GDNF; HSCR3; mGDNF; neurotrophic factor
Common Name GDNF
Gene Symbol GDNF
Biological Activity Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml
Conjugate Unconjugated
Recombinant Recombinant
Sequence MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Content And Storage -20°C
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 98% by SDS-PAGE gel and HPLC analyses.
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