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  5. Invitrogen™ Human Flt-3 ELISA Kit

Invitrogen™ Human Flt-3 ELISA Kit

Catalog No. EH197RBX5
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5 x 96 Tests
96 Tests
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EH197RBX5 5 x 96 Tests
EH197RB 96 Tests
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Description

ELISA

Human Flt-3 quantitates human Flt-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Flt-3.

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Specifications

Accession Number B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; Flk2; Flk-2; Flt3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl
Assay Range 0.246 to 60 ng/mL
Assay Sensitivity 0.24 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2322
Gene Symbol FLT3
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target FLT3 (CD135)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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