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Invitrogen™ Human Ferritin Heavy Polypeptide ELISA Kit

Catalog No. EEL183
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96 Tests
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Catalog No. Quantity
EEL183 96 Tests
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Catalog No. EEL183 Supplier Invitrogen™ Supplier No. EEL183
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Description

ELISA

FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.

Specifications

Accession Number P02794
Assay Range 12.5 to 800 ng/mL
Assay Sensitivity 7.5 ng/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2495
Gene Symbol FTH1
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Ferritin Heavy Chain
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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