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Gibco™ Human EGF Recombinant Protein

Catalog No. phg0314
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10 μg
100 μg
25 μg
1 mg
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Catalog No. Quantity
PHG0314 10 μg
PHG0311 100 μg
PHG0313 1 mg
PHG0315 25 μg
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Catalog No. PHG0314 Supplier Gibco™ Supplier No. PHG0314
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Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

Accession Number P01133
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 1950
Molecular Weight (g/mol) 6.2 kDa
Name Human EGF
Purification Method Purified
Quantity 10 μg
Storage Requirements 4°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
Common Name EGF
Gene Symbol EGF
Biological Activity ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Classification Carrier-Free
Activity ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype EGF (Epidermal Growth Factor)
Research Category Stem Cell Research, Oncology
Product Line Gibco
Purity or Quality Grade >95% by SDS-PAGE
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