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Invitrogen™ Human ECSIT (aa 233-330) Control Fragment Recombinant Protein

Catalog No. RP99074
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100 μL
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Catalog No. Quantity
RP99074 100 μL
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Catalog No. RP99074 Supplier Invitrogen™ Supplier No. RP99074

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (84%), Rat (84%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60183 (PA5-60183. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Specifications

Accession Number Q9BQ95
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 51295
Name Human ECSIT (aa 233-330) Control Fragment
Quantity 100 μL
Gene Alias Ecsit; ECSIT homolog; ECSIT homolog (Drosophila); ECSIT signalling integrator; evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved; Protein SITPEC; signaling intermediate in Toll pathway-evolutionarily conserved; Sitpec
Common Name ECSIT
Gene Symbol ECSIT
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence HMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPM
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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