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  5. Invitrogen™ Human Desmin ELISA Kit

Invitrogen™ Human Desmin ELISA Kit

Catalog No. EH152RBX5
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5 x 96 Tests
96 Tests
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EH152RBX5 5 x 96 Tests
EH152RB 96 Tests
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Description

ELISA

Human Desmin quantitates human Desmin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Desmin.

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Specifications

Accession Number P17661
Assay Range 2-500 ng/mL
Assay Sensitivity 2 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias CMD1I; CSM1; CSM2; DES; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; I79_018932; intermediate filament protein; LGMD2R; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK; similar to desminCMD1I; CSM1; CSM2; DES; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; I79_018932; intermediate filament protein; LGMD2R; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK; similar to desmin;
Gene ID (Entrez) 1674
Gene Symbol DES
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target Desmin
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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