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Invitrogen™ Human CYP7B1 (aa 318-449) Control Fragment Recombinant Protein

Catalog No. RP91048
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100 μL
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RP91048 100 μL
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Catalog No. RP91048 Supplier Invitrogen™ Supplier No. RP91048

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (70%), Rat (70%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53639 (PA5-53639. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Specifications

Accession Number O75881
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 9420
Name Human CYP7B1 (aa 318-449) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 24-hydroxycholesterol 7-alpha-hydroxylase; 25/26-hydroxycholesterol 7-alpha-hydroxylase; 25-hydroxycholesterol 7-alpha-hydroxylase; 3-hydroxysteroid 7-alpha hydroxylase; AW261589; CBAS3; CP7B; CYP7B1; Cytochrome P450 7B1; cytochrome P450 family 7 subfamily B member 1; cytochrome P450, 7b1; cytochrome P450, family 7, subfamily b, polypeptide 1; cytochrome P450, subfamily 7 B, polypeptide 1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; D3Ertd552e; hct-1; hippocampal transcript 1 protein; Oxysterol 7-alpha-hydroxylase; SPG5A
Common Name CYP7B1
Gene Symbol Cyp7b1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence VRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKC
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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