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  5. Invitrogen™ Human Coagulation Factor VII ELISA Kit

Invitrogen™ Human Coagulation Factor VII ELISA Kit

Catalog No. EH117RBX5
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5 x 96 Tests
96 Tests
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EH117RBX5 5 x 96 Tests
EH117RB 96 Tests
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Description

ELISA

Human Coagulation Factor VII quantitates human Coagulation Factor VII in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Coagulation Factor VII.

Coagulation factor VII is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in F7 production can cause coagulopathy.

Specifications

Accession Number AI132620; Cf7; Coagulation factor VII; coagulation factor VII (serum prothrombin conversion accelerator); eptacog alfa; F7; Factor 7; Factor VII heavy chain; Factor VII light chain; Factor7; FVII; FVII coagulation protein; proconvertin; Serum prothrombin conversion accelerator; SPCA
Assay Range 0.4 to 100 ng/mL
Assay Sensitivity 0.4 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 2155
Gene Symbol F7
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Factor VII
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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