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  5. Invitrogen™ Human CHST4/GST-3 ELISA Kit

Invitrogen™ Human CHST4/GST-3 ELISA Kit

Catalog No. EH107RBX5
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Description

ELISA

Human CHST4/GST-3 quantitates human CHST4/GST-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CHST4/GST-3.

This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.

Specifications

Accession Number carbohydrate (chondroitin 6/keratan) sulfotransferase 4; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; carbohydrate sulfotransferase 4; CHST4; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; Gn6st-2; Gst3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; high endothelial cell GlcNAC-6-sulphotransferase; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2
Assay Range 0.062 to 150 ng/mL
Assay Sensitivity 0.062 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 10164
Gene Symbol Chst4
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CHST4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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