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  5. Invitrogen™ Human Cathepsin H ELISA Kit

Invitrogen™ Human Cathepsin H ELISA Kit

Catalog No. EH75RBX5
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5 x 96 Tests
96 Tests
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EH75RBX5 5 x 96 Tests
EH75RB 96 Tests
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Description

ELISA

Human Cathepsin H quantitates human Cathepsin H in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Cathepsin H.

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer.

Specifications

Accession Number P09668
Assay Range 0.17-40 ng/mL
Assay Sensitivity 0.17 ng/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias ACC4; ACC-4; ACC5; ACC-5; AL022844; aleurain; Cat H; CATH; cathepsin B3; Cathepsin BA; cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; Cathepsin H mini chain; CPSB; Ctsh; N-benzoylarginine-beta-naphthylamide hydrolase; pro-cathepsin HACC4; ACC-4; ACC5; ACC-5; AL022844; aleurain; Cat H; CATH; cathepsin B3; Cathepsin BA; cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; Cathepsin H mini chain; CPSB; Ctsh; N-benzoylarginine-beta-naphthylamide hydrolase; pro-cathepsin H;
Gene ID (Entrez) 1512
Gene Symbol CTSH
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target Cathepsin H
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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