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  5. Invitrogen™ Human Carboxypeptidase A1/CPA1 ELISA Kit

Invitrogen™ Human Carboxypeptidase A1/CPA1 ELISA Kit

Catalog No. EH66RBX5
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EH66RBX5 5 x 96 Tests
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Description

ELISA

Human Carboxypeptidase A1/CPA1 quantitates human Carboxypeptidase A1/CPA1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Carboxypeptidase A1/CPA1.

CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.

Specifications

Accession Number 0910001L12Rik; carboxypeptidase A; carboxypeptidase A1; carboxypeptidase A1 (pancreatic); carboxypeptidase A1, pancreatic; Cpa; CPA1; pancreatic carboxypeptidase A; pancreatic procarboxypeptidase A
Assay Range 0.65 to 150 ng/mL
Assay Sensitivity 0.65 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1357
Gene Symbol Cpa1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Carboxypeptidase A1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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