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  5. Invitrogen™ Human Annexin V ELISA Kit

Invitrogen™ Human Annexin V ELISA Kit

Catalog No. 5018168
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96 Tests
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Catalog No. Quantity
5018168 96 Tests
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Catalog No. 5018168 Supplier Invitrogen™ Supplier No. BMS252

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
Biotin-Conjugate anti-human Annexin V monoclonal antibody
Streptavidin-HRP
Human Annexin V Standard lyophilized, 100ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

ELISA

The Human Annexin V ELISA quantitates Hu Annexin V in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Annexin V. Principle of the method The Human Annexin V solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Specifications

Accession Number P08758
Assay Range 0.78-50 ng/mL
Assay Sensitivity 0.33 ng/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human Annexin V
Biotin-Conjugate anti-human Annexin V monoclonal antibody
Streptavidin-HRP
Human Annexin V Standard lyophilized, 100 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Conjugate HRP
Detection Method Colorimetric
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias Anchorin CII; annexin 5; annexin A5; Annexin V; annexin-5; Anx5; ANXA5; Calphobindin I; CBP-I; Endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; LC5; lipocortin V; PAP-I; placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; R74653; RPRGL3; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alphaAnchorin CII; annexin 5; annexin A5; Annexin V; annexin-5; Anx5; ANXA5; Calphobindin I; CBP-I; Endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; LC5; lipocortin V; PAP-I; placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; R74653; RPRGL3; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha; ANX5; ENX2; HEL-S-7; PP4; RPRGL3
Gene ID (Entrez) 308
Gene Symbol Anxa5
Interassay CV 11%
Intraassay CV 10%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Storage Requirements 2-8°C
Synonym Lipocortin V, Endonexin II, Calphobindin I
Target Annexin V
Test Time 1 hr 20 min
Total Assay Time 3 hr 10 min
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Frequently Asked Questions (FAQs)

Do you have information regarding the normal values detectable with your Platinum ELISA Kit?

You can find expected "normal levels" listed in the Platinum ELISA Kit manual under "Expected Values". Please be aware that these normal values were obtained in our laboratories with a limited number of samples. We strongly suggest that each laboratory perform studies in order to establish normal range for their sample populations.
What if it does not look like the supplied reagent volume is sufficient for running the Platinum ELISA?

In case of small reagent volumes, please spin down vial before use to make sure to all contents are collected in the bottom of the tube.
Can I omit the dilution of my Platinum ELISA samples with assay buffer/sample diluent when performing the assay?

We recommend following the instructions for sample dilutions as provided in the manual. We cannot guarantee optimal performance of the assay if internal assay dilutions as instructed were omitted.
Are shorter or longer incubation times possible when using your Platinum ELISA kit?

Incubation times should be followed exactly as stated in the product manual to ensure optimal Platinum ELISA test performance.
Is it necessary to wash the Platinum ELISA plates before usage?

An initial washing step is not necessary but recommended as accuracy may be increased.
Can I always use the same manual for different Platinum ELISA kit lot numbers?

We recommend that you use the manual included with the Platinum ELISA kit.
Is it possible to use the reagents of other Platinum ELISA kits/lots?

The reagents within the Platinum ELISA kits are not interchangeable between different lots of the same kit or between kits against different analytes.
Do I have to run the Platinum ELISA standards and samples in duplicates?

No, but we strongly recommend that you make at least double determinations of each sample and standard point.
Which samples are suitable for Platinum ELISA Kits?

Most ELISAs are suitable for serum, plasma, cell culture supernatants and other body fluids. Detailed information is given in the product manual for each individual kit.
What do Platinum ELISA Kits contain?

All reagents necessary to perform 1 or 10 plate ELISA assays, including optimized capture-antibody pre-coated plates.
I want to collect my tissue culture supernatant daily for a time-course study and run all of my samples at once by ELISA. How should I store my supernatants for use with the eBioscience Platinum ELISA kits?

The supernatants can be harvested and stored -20 degrees C. Avoid freeze/thaw cycles. We recommend that you briefly centrifuge samples after collection to pellet any dead/floating cells remaining in the supernatant. Transfer to new tube for storage.
What concentrations should I use for my recombinant standard when using the eBioscience Platinum ELISA kits?

Each Ready-Set-Go! ELISA Set comes with its own Certificate of Analysis (CofA). The directions on the CofA should be strictly followed to ensure performance of the reagents. The CofA gives specific instructions on how to dilute each component of your lot specific set. The instructions on the CofA should not be deviated from in order to ensure accurate results.
How do I calculate my samples when using the eBioscience Platinum ELISA kits?

The calculation factor is always stated in the manual.
Is the reconstitution volume of the standards the same for different lot numbers when using the eBioscience Platinum ELISA kits?

For some Instant ELISA kits, the reconstitution volume of the standard and blank wells may differ (these components are lot specific). Please use the manual provided in the kit for specific volumes to use.
Do I have to dilute my samples before adding them to the wells when using the eBioscience Platinum ELISA kits?

In general, you can use undiluted samples with the Instant ELISA. Please refer to the product manual to determine if dilution of the sample is required for your kit of interest. The volume of sample required for the individual kits will also be listed in the product manual.
For the eBioscience Platinum ELISA kits, do I have to add the secondary antibody to the wells?

No, the secondary antibody is included as a component of the lyophilized reagents in the wells.
For the eBioscience Platinum ELISA kits, do I have to add recombinant standard to the wells?

No, the recombinant standard is included as a component of the lyophilized reagents in the wells.
Is it possible to store the reagents from the eBioscience Platinum ELISA Kits other than indicated?

Performance of Platinum ELISA cannot be guaranteed if reagents are not stored as indicated.