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  5. Invitrogen™ Human Adiponectin ELISA Kit

Invitrogen™ Human Adiponectin ELISA Kit

Catalog No. 501125260
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96 Tests
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Catalog No. Quantity
501125260 96 Tests
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Catalog No. 501125260 Supplier Invitrogen™ Supplier No. BMS20322
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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
Biotin-Conjugate anti-human Adiponectin monoclonal antibody
Streptavidin-HRP
Human Adiponectin Standard lyophilized, 30ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

ELISA

The Human Adiponectin ELISA quantitates Hu Adiponectin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Adiponectin. Principle of the method The Human Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

Specifications

Accession Number Q15848
Assay Range 0.23-15 ng/mL
Assay Sensitivity 0.012 ng/mL
Conjugate HRP
Detection Method Colorimetric
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 30 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 2830 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 28; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
Gene ID (Entrez) 9370
Gene Symbol ADIPOQ
Interassay CV 3.1%
Intraassay CV 4.2%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Storage Requirements 2-8°C
Synonym ACDC, apM1, GBP-28, ADIPOQ
Target Adiponectin
Test Time 1 hr 20 min
Total Assay Time 3 hr 30 min
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