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Cytiva HiTrap™ TALON™ Crude Prepacked Columns

Catalog No. p-4415925
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Quantity:
1 x 1 mL
5 x 1 mL
5 x 5 mL
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Catalog No. Quantity
45002386 5 x 5 mL
45002385 5 x 1 mL
45000502 1 x 1 mL
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Catalog No. 45002386 Supplier Cytiva Supplier No. 28953767
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Description

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

Specifications

Quantity 5 x 5 mL
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Storage Requirements 4°C to 8°C
Binding Capacity Up to 20 mg Histidine-tagged Protein1
Diameter (English) Inner 0.62 in.
Product Type HiTrap Talon crude prepacked Column
Diameter (Metric) Inner 1.6 cm
Flow Rate 1 to 4 mL/min
Particle Size 60 to 160 μm
Material Polypropylene
Max. Pressure 3 bar
Matrix 6% Cross-Linked Agarose
pH Range 3 to 12
Storage Buffer 20% Ethanol
Ligand Type Talon Superflow Cobalt-IMAC
Packaging Quantity 5 x 5 mL
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