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Cytiva HiTrap™ Protein G HP Prepacked Columns

Catalog No. 45000054
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Binding Capacity:
125 mg Human IgG/Column
25 mg Human IgG/Column
Quantity:
1 Pk.
2 Pk.
5 Pk.
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Catalog No. Binding Capacity Quantity
45000054 25 mg Human IgG/Column 2 Pk.
45000053 25 mg Human IgG/Column 5 Pk.
45000055 125 mg Human IgG/Column 1 Pk.
45000056 125 mg Human IgG/Column 5 Pk.
45000504 25 mg Human IgG/Column 1 Pk.
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Catalog No. 45000054 Supplier Cytiva Supplier No. 17040403
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Description

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

  • Prepacked with Protein G Sepharose High Performance
  • Binding capacity: 25mg human IgG/column
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • For use with syringe, peristaltic pump, or chromatography system
  • 1 and 5mL columns

Media
  • Ligand: Recombinant protein G lacking albumin-binding region
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 9

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Product Type Pre-packed Columns
Binding Capacity 25 mg Human IgG/Column
Diameter (Metric) Inner Column 7 mm
Diameter (English) Inner Column 0.27 in.
Flow Rate <4 mL/min.
Volume (Metric) Bed 1 mL
For Use With (Application) Designed for use with a syringe, peristaltic pump, or chromatography system
Description HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
Storage Buffer 20% Ethanol
Content And Storage 4°C to 30°C
Quantity 2 Pk.
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