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Cytiva HiTrap™ Protein A HP Prepacked Columns

Catalog No. 45000050
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Quantity:
1 x 1 mL
1 x 5 mL
2 x 1 mL
5 x 1 mL
5 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
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Catalog No. Quantity Flow Rate
45000050 2 x 1 mL <4 mL/min.
45000052 5 x 5 mL <20 mL/min.
45000049 5 x 1 mL <4 mL/min.
45000051 1 x 5 mL <20 mL/min.
45000503 1 x 1 mL <4 mL/min.
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Catalog No. 45000050 Supplier Cytiva Supplier No. 17040203
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Description

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

  • Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
  • For use with syringe, pump or chromatography system such as KTA design or FPLC System
  • Binds 20mg human IgG/mL medium
  • Stable over pH 3 to 9 for use and 2 to 10 for cleaning
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns

Media
  • Ligand: Native protein A
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 9

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Binding Capacity 20 mg/mL IgG Medium
Flow Rate <4 mL/min.
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 8°C, 20% Ethanol
Quantity 2 x 1 mL