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Invitrogen™ Histamine Competitive ELISA Kit

Catalog No. EEL005
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96 Tests
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Catalog No. Quantity
EEL005 96 Tests
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Catalog No. EEL005 Supplier Invitrogen™ Supplier No. EEL005
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Description

ELISA

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cell found in various tissues as a result of allergic hytersensitivity or inflammation. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain.

Specifications

Assay Range 1.56 to 100 ng/mL
Assay Sensitivity 0.94 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Histamine
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 30 mins
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