Thermo Scientific™ HindIII (10 U/μL)

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

Supplier: Thermo Scientific™ ER0502

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Numéro de catalogue. FERER0502

$255.32 / chaque

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Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

Superior quality – stringent quality control and industry leading manufacturing process
Convenient color-coded Five Buffer System
Includes universal Tango buffer for double-digestions
BSA premixed in reaction buffers
Wide selection of restriction endonuclease specificities

Applications

Molecular cloning
Restriction site mapping
Genotyping
Southern Blot
Restriction fragment length polymorphism (RFLP)
SNP

Spécifications

Concentration	10 U/μL
Enzyme	Hind III
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	10X Buffer R
Optimal Reaction Temperature	37°C
Quantity	5 x 5,000 units
Research Category	Traditional Cloning

missing translation for 'recommendations'

For Research Use Only. Not for use in diagnostic procedures.