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  5. Invitrogen™ Mouse HGF-Cell Lysates ELISA Kit

Invitrogen™ Mouse HGF-Cell Lysates ELISA Kit

Catalog No. EMHGFCL
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96 Tests
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Catalog No. Quantity
EMHGFCL 96 Tests
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Catalog No. EMHGFCL Supplier Invitrogen™ Supplier No. EMHGFCL

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Description

ELISA

The Mouse Hepatocyte Growth Factor(Ms HGF) Cell Lysates ELISA quantitates Ms HGF in mouse cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

Specifications

Accession Number Q08048
Assay Range 400-60 ng/mL
Assay Sensitivity 400 pg/mL
Calibration Range 400 pg/mL to 60 ng/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Chemokines & Receptors
Sample Type Cell Lysates, Plasma, Serum, Supernatant, Tissue Homogenates
Specificity The antibody pair provided in this kit recognizes mouse HGF.
Sufficient For 96 Assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias C230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGFC230052L06Rik; DFNB39; Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; hepatopoeitin-A; HepatopoietinA; hepatopoietin-A; Hgf; HGF/SF; HGFB; HPTA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; NK1; NK2; Scatter factor; scatter factor); SF; SF/HGF; C230052L06Rik; HGF/SF; NK1; NK2; SF; SF/HGF
Gene ID (Entrez) 15234
Gene Symbol Hgf
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases Hepatopoietin-A, Scatter factor, SF
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Apoptosis
Sample Volume Cell Lysate 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF
Target HGF
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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