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Invitrogen™ HCS CellMask™ Stains
Description
Identify entire cells (cytoplasm and nucleus) during high-content screening (HCS) assays with the versatile HCS CellMask Red, CellMask Orange, CellMask Green, CellMask Blue, CellMask Deep Red, and CellMask Near-IR stains, which are fluorophores that can be used immediately after fixation and permeabilization, or after antibody labeling. They are available in a variety of colors for multiplexing protocols.
HCS CellMask stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask stains can be used for measuring DNA content in addition to enabling robust cell demarcation. HCS NuclearMask stains are also available in a choice of colors for multiplexing flexibility.
Specifications
Specifications
| Content And Storage | Includes 1 set of vials, providing sufficient material to stain a total of ten 96-well microplates. Store at ≤-20°C, desiccate, and protect from light. |
| Format | Vial(s) |
| For Use With (Equipment) | High Content Instrument |
| Quantity | 1 Set |
| Detection Method | Fluorescence |
| Description | HCS CellMask Near-IR Stain |
| Product Line | CellMask |
| Shipping Condition | Room Temperature |
| Label Type | Fluorescent Dye |
| Product Type | Stain |
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Frequently Asked Questions (FAQs)
As shipped, the product has a warranty period of 1 year from the date of shipment. Once the solid stain is resolubilized in DMSO, it should be stored frozen, desiccated, and protected from light and is useable for ~ 6 months.
Each HCS CellMask Stain provides sufficient material for labeling approximately 10 x 96-well plates using an assay volume of 100 µL. The product is shipped as two components: a single vial of 250 µg of solid Near-IR Stain (Component A) and a single vial of 100 µL of DMSO (Component B).
We recommend storing Component A at ≤ -20 degrees C, desiccated and protected from light and Component B desiccated at ≤ 25 degrees C.
The proprietary fluorescent dyes in the CellMask stains are general cytoplasmic stains. They are not found to bind to any specific cellular component.