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  5. Invitrogen™ Mouse gp130 (IL 6ST) ELISA Kit

Invitrogen™ Mouse gp130 (IL 6ST) ELISA Kit

Catalog No. EMIL6ST
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96 Tests
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Catalog No. Quantity
EMIL6ST 96 Tests
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Catalog No. EMIL6ST Supplier Invitrogen™ Supplier No. EMIL6ST

Description

ELISA

The Mouse gp130 (IL-6ST) ELISA quantitates Ms gp130 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms gp130. Principle of the method The Mouse gp130 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Specifications

Accession Number Q00560
Assay Range 40.96 to 10,000 pg/mL
Assay Sensitivity 40 pg/mL
Calibration Range 40 pg/mL to 10000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detects human mouse gp-130. Other species not determined yet
Sufficient For 96 assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16195
Gene Symbol 5133400A03Rik,AA389424,BB405851,CD130,D13Ertd699e,gp130
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Oncology
Sample Volume Plasma 3 μL, Serum 3 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym 5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
Target GP130
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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