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Invitrogen™ Goat anti-Rat IgG (H+L) Secondary Antibody, NovaFluor™ Yellow 590, eBioscience™

Catalog No. S002T03Y02A
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100 μg
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S002T03Y02A 100 μg
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Catalog No. S002T03Y02A Supplier Invitrogen™ Supplier No. S002T03Y02A

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Description

Goat Polyclonal Secondary Antibody

Description: This is affinity purified HRP-conjugated Goat Anti-Rat IgG (H+L). This antibody has minimal cross-reactivity to Mouse, Human, Bovine, Horse, and Rabbit Serum Proteins. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rat IgG and with thelight chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer . Applications Reported: HRP anti-rat IgG has been reported for use in immunoblotting (WB), immunohistochemical staining, and ELISA. Applications Tested: This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human,bovine, horse, and rabbit serum proteins, but the antibody may cross-react with immunoglobulins from other species.Suggested dilution ranges are:1:500 - 1:5,000 for immunohistochemistry on tissue sections 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains ...

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG (H+L)
Applications Flow Cytometry
Classification Polyclonal
Concentration 0.1 mg/mL
Conjugate NovaFluor Yellow 590
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Goat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Format Liquid
Isotype Ig
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