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Invitrogen™ Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 633

Catalog No. PI35563
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500 μL
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Catalog No. Quantity
PI35563 500 μL
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Catalog No. PI35563 Supplier Invitrogen™ Supplier No. 35563

Description

Goat Polyclonal Secondary Antibody

Product # 35563 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 35563 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by solid-phase adsorbed to ensure minimal cross-reactivity with human, horse, mouse, swine, goat, bovine, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 633 Amax= 638 nm; Emax= 658 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rabbit IgG (H+L) Cross-Adsorbed
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate DyLight 633
Formulation PBS with 0.02% sodium azide; pH 7.2
Host Species Goat
Purification Method Antigen affinity chromatography
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rabbit
Content And Storage 4°C
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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