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Invitrogen™ Goat anti-Mouse IgG1 Secondary Antibody, PE-Cyanine5.5
Description
Goat anti-Mouse IgG1 Secondary Antibody for Flow
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications
Specifications
| Antigen | Mouse IgG1 |
| Applications | Flow Cytometry |
| Classification | Polyclonal |
| Conjugate | PE-Cyanine5.5 |
| Formulation | PBS with sucrose, 4mg/mL BSA and 0.1% sodium azide |
| Host Species | Goat |
| Immunogen | IgG gamma 1. |
| Purification Method | Purified |
| Quantity | 500 μL |
| Regulatory Status | RUO |
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Frequently Asked Questions (FAQs)
I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?
If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.