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Invitrogen™ Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, PE

Catalog No. 5000555621
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500 μL
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5000555621 500 μL
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Catalog No. 5000555621 Supplier Invitrogen™ Supplier No. SA510385PE
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Description

Goat Recombinant Superclonal Secondary Antibody

Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, PE specifically detects mouse IgG with no cross-reactivity to immunoglobulins tested from other species. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG (H+L)
Applications Flow Cytometry
Classification Recombinant Superclonal
Concentration 0.2 mg/mL
Conjugate PE
Formulation PBS with 5mM sodium azide; pH 7.4
Host Species Goat
Immunogen Purified Mouse IgG.
Purification Method Affinity chromatography
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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