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Invitrogen™ Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 647

Catalog No. PIA28181
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PIA28181 1 mg
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Catalog No. PIA28181 Supplier Invitrogen™ Supplier No. A28181
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Goat Recombinant Superclonal Secondary Antibody

The sensitivity and specificity of each lot is confirmed using Immunocytochemistry. Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG (H+L)
Applications Western Blot, Immunocytochemistry
Classification Recombinant Superclonal
Concentration 1 mg/mL
Conjugate Alexa Fluor 647
Formulation PBS with 5mM sodium azide
Host Species Goat
Immunogen Recombinant full-length protein.
Purification Method Gravity column chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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