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  6. Invitrogen™ Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody, HRP

Invitrogen™ Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody, HRP

Catalog No. A5573950UL
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Quantity:
50 μL
250 μL
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Catalog No. Quantity
A5573950UL 50 μL
A55739 250 μL
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Description

Goat Recombinant Monoclonal Secondary Antibody

This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody specifically detects human IgG1 heavy chain with no cross-reactivity to human IgG2, IgG3, IgG4, IgA or IgM or various species immunoglobulins, including monkey IgG. The sensitivity and specificity of each lot is confirmed using ELISA. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Human IgG1 (Heavy chain)
Applications Peptide Array, ELISA, Western Blot
Classification Recombinant Monoclonal
Clone 3H60L9
Concentration 1.0 mg/mL
Conjugate HRP
Formulation PBS with proprietary stabilizer and proprietary preservative; pH 7.4
Host Species Goat
Immunogen Purified Human IgG.
Purification Method Protein G
Quantity 50 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Human
Content And Storage 4°C, do not freeze
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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