Invitrogen™ Goat anti-Human IgG F(ab')2 Secondary Antibody, Rhodamine

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product # 31684 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31684 reacts with the light chains of human IgG and with those common to other human immunoglobulins, but does not react against the Fc portion of human IgG or against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein Absorbance Ratio: A550/A280 = ~ 0.5 (lot-dependent). Country of Origin: USA.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.