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Invitrogen™ Goat anti-Armenian Hamster IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568
Description
This antibody specifically detects Armenian hamster IgG and shows negligible reactivity to Syrian hamster IgG as tested by ELISA and immunofluorescence. This goat anti-armenian hamster IgG (H+L) whole secondary antibody has been affinity-purified and shows minimum cross-reactivity to bovine, human, mouse, rabbit, rat serum proteins.. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondary antibodies flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g. flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 568 dye is a bright, orange/red-fluorescent dye with excitation ideally suited to the 568 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detectio...
Specifications
Specifications
| Antigen | Armenian Hamster IgG (H+L) Highly Cross-Adsorbed |
| Applications | Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry |
| Classification | Polyclonal |
| Concentration | 2 mg/mL |
| Conjugate | Alexa Fluor 568 |
| Formulation | PBS with 5mM sodium azide; pH 7.5 |
| Host Species | Goat |
| Immunogen | Gamma Immunoglobins Heavy and Light chains. |
| Purification Method | Purified |
| Quantity | 500 μg |
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