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Invitrogen™ Glutathione Competitive ELISA Kit
Description
Glutathione is a tripeptide comprised of three amino acids (cysteine, glutamic acid, and glycine) present in most mammalian tissue. Glutathione acts as an antioxidant, a free radical scavenger and a detoxifying agent. Glutathione is also important as a cofactor for the enzyme glutathione peroxidase, in the uptake of amino acids, and in the synthesis of leukotrienes. As a substrate for glutathione S-transferase, this agent reacts with a number of harmful chemical species, such as halides, epoxides and free radicals, to form harmless inactive products. In erythrocytes, these reactions prevent oxidative damage through the reduction of methemoglobin and peroxides. Glutathione is also involved in the formation and maintenance of disulfide bonds in proteins and in the transport of amino acids across cell membranes.
Specifications
Specifications
| Assay Range | 1.56 to 100 μg/mL |
| Assay Sensitivity | 0.94 μg/mL |
| Conjugate | Unlabeled |
| Product Type | ELISA |
| Sample Type | Cell Lysates, Plasma, Serum, Tissue Homogenates |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Interassay CV | <10 |
| Intraassay CV | <10 |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer |
| Label or Dye | Biotin |
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