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Invitrogen™ Glutathione Competitive ELISA Kit

Catalog No. EEL155
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96 Tests
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Catalog No. Quantity
EEL155 96 Tests
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Catalog No. EEL155 Supplier Invitrogen™ Supplier No. EEL155

ELISA

Glutathione is a tripeptide comprised of three amino acids (cysteine, glutamic acid, and glycine) present in most mammalian tissue. Glutathione acts as an antioxidant, a free radical scavenger and a detoxifying agent. Glutathione is also important as a cofactor for the enzyme glutathione peroxidase, in the uptake of amino acids, and in the synthesis of leukotrienes. As a substrate for glutathione S-transferase, this agent reacts with a number of harmful chemical species, such as halides, epoxides and free radicals, to form harmless inactive products. In erythrocytes, these reactions prevent oxidative damage through the reduction of methemoglobin and peroxides. Glutathione is also involved in the formation and maintenance of disulfide bonds in proteins and in the transport of amino acids across cell membranes.

Specifications

Assay Range 1.56 to 100 μg/mL
Assay Sensitivity 0.94 μg/mL
Conjugate Unlabeled
Product Type ELISA
Sample Type Cell Lysates, Plasma, Serum, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye Biotin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Glutathione
Test Time 1 hrs 20 mins
Total Assay Time 2 hrs 30 mins
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