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Invitrogen™ Human GITR ELISA Kit

Catalog No. BMS2210
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96 Tests
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BMS2210 96 Tests
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Catalog No. BMS2210 Supplier Invitrogen™ Supplier No. BMS2210

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Description

ELISA

The Human Glucocorticoid-Induced TNFR-Related protein (Hu GITR) ELISA quantitates Hu GITR in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GITR. Principle of the method The Human GITR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Specifications

Accession Number Q9Y5U5
Assay Range 62.3 to 4,000 pg/mL
Assay Sensitivity 9.7 pg/ml
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 8784
Gene Symbol TNFSF18,AITRL,AITR,TL6,CD357 Antigen,GITR-D,CD357
Interassay CV 8.1%
Intraassay CV 3.3
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target GITR
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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