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Gibco™ Sodium Bicarbonate 7.5% solution
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Catalog No. 25080094
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25080094 100 mL
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Catalog No. 25080094 Supplier Gibco™ Supplier No. 25080094

Description

Commonly used for maintaining the pH of cell culture medium in the presence of 4 to 10% carbon dioxide

  • Addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity
  • Formulated as 75g/L of water, excellent for supplementing dry powder medium during reconstitution
  • Final sodium bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator
  • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
Cell Type Mammalian Cells
Concentration 0.075
Form Liquid
Product Type Sodium Bicarbonate
Sterility Sterile-filtered
pH 8.3
Quantity 100 mL
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Based on the Sodium bicarbonate levels in the medium, what CO2 % is recommended?

If the media formulation contains:

NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .

*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?

Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.

If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.

If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.

*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

For Research Use Only. Not for use in diagnostic procedures.