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Gibco™ RPMI 1640 Medium, Powder, HEPES DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

$77.70 - $109.20

Specifications

Format Powder
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
View More Specs
Requires:  Supplementation

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

23400062

DFS Item
 
life technologies
23400062
10L Each for $77.70

23400021

DFS Item
 
life technologies
23400021
10 x 1L Each for $109.20
Description & Specifications

Specifications

Format Powder
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, HEPES, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture

Roswell Park Memorial Institute (RPMI) 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas.

  • RPMI is modified as follows: with L-glutamine, phenol red, HEPES; without sodium bicarbonate
  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in very high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

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