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Gibco™ RPMI 1640 Medium, powder, HEPES

Catalog No. 23400062
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Catalog No. 23400062 Supplier Gibco™ Supplier No. 23400062
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Description

Requires

Supplementation

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.

This RPMI is modified as follows:

With: L-glutamine, Phenol Red, HEPES

Without: Sodium Bicarbonate

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type Leukemic Cells
Classification Animal Origin-free
Form Powder
Product Type RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives Glutamine, HEPES, Phenol Red
Without Additives No Sodium Pyruvate, No Sodium Bicarbonate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 10 L
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.