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Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

$72.75 - $1,068.00


With Additives GlutaMAX-I, Phenol Red, HEPES
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Requires: Supplementation

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
72400047 DFS Item
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Each for $72.75
72400120 DFS Item
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10 x 500mL
Each for $1,068.00


RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Modified as follows: with GlutaMAX™, phenol red, HEPES. RPMI 1640 medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature



GlutaMAX-I, Phenol Red, HEPES
Product Certifications

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.