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Gibco™ RPMI 1640 Medium DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

$10.02 - $357.00

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs
Requires:  Supplementation

 Disclaimers

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

11875127

DFS Item
 
life technologies
11875127
20 x 100mL Each for $172.20

11875101

DFS Item
 
life technologies
11875101
100mL Each for $10.02

11875085

DFS Item
 
life technologies
11875085
1000mL Each for $67.73

11875119

DFS Item
 
life technologies
11875119
10 x 500mL Each for $349.65

11875093

DFS Item
 
life technologies
11875093
500mL Each for $39.55

11875135

DFS Item
 
life technologies
11875135
6 x 1000mL Each for $357.00
Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Storage Requirements Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture

Roswell Park Memorial Institute (RPMI) 1640 Medium is suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • RPMI is modified as follows: with L-glutamine, phenol red; without HEPES
  • Unique from other media because it contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Shipping Condition: Room Temperature

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