missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Classical and Basal Cell Culture Media
  6. RPMI Cell Culture Media
  7. Gibco™ RPMI 1640 Medium

Gibco™ RPMI 1640 Medium
GREENER_CHOICE

Catalog No. 11875101
Encompass
Change view
Click to view available options
Quantity:
100 mL
500 mL
1000 mL
10 x 500 mL
20 x 100 mL
6 x 1000 mL
6 product options available for selection
Product selection table with 6 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
11875101 100 mL
11875127 20 x 100 mL
11875093 500 mL
11875119 10 x 500 mL
11875085 1000 mL
11875135 6 x 1000 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 6 options available.
6 options
Catalog No. 11875101 Supplier Gibco™ Supplier No. 11875101
Only null left

Description

Requires

Supplementation

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using RPMI

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Cell Line HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type Leukemic Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Glutamine, Phenol Red
Without Additives No HEPES, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 100 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Show More Show Less

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.