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Gibco™ Penicillin-Streptomycin-Glutamine (100X)
Description
Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that alter cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria.
- Available as 100 mL liquid
- Solution contains 10,000 units of penicillin, 10,000μg of streptomycin, and 29.2 mg/mL of L-glutamine in a 10 mM citrate buffer (for pH stability)
Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Content And Storage | Storage conditions: -5°C to -20°C Shipping conditions: Dry ice Shelf life: 12 months from date of manufacture |
| Concentration | 100 X, 50 mg/mL |
| Form | Liquid |
| Product Type | Antibiotic-L-Glutamine |
| Sterility | Sterile-filtered |
| With Additives | Glutamine |
| For Use With (Application) | Prevention of Cell Culture Contamination |
| Quantity | 100 mL |
| Shipping Condition | Dry Ice |
Frequently Asked Questions (FAQs)
The Penicillin-Streptomycin-Glutamine solution is at 100X. This can be used at a 1X concentration in media.
Yes, this is still good to use. The precipitate is due to the glutamine. The solution needs to be warmed to room temp and swirled unitl the glutamine goes back into solution.
Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.
This contains: Penicillin G Sodium at 12,000 Units/mL, Streptomycin Sulfate at 10,000 mcg/mL, L-glutamine at 29.2 mg/mL (200 mM), and Sodium Citrate 0.14% NaCl at 10mM
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
For Research Use Only. Not for use in diagnostic procedures.