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Gibco™ HAT Supplement (50X)
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Catalog No. 21060017
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100 mL
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21060017 100 mL
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Catalog No. 21060017 Supplier Gibco™ Supplier No. 21060017
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Description

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

  • Liquid mixture of sodium hypoxanthine (5mM), aminopterin (20μM) and thymidine (0.8mM)
  • Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT contributed by the fused spleen cell
  • Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Dry Ice

Specifications

Content And Storage Storage conditions: -5 to -20°C
Shipping conditions: Dry ice
Shelf life: 14 months from date of manufacture
Cell Type Mammalian Cells
Concentration 50 X
Form Liquid
Product Type HT Supplement
Sterility Sterile-filtered
pH 6 to 8
Quantity 100 mL
Shipping Condition Dry Ice

For Research Use Only. Not for use in diagnostic procedures.