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Gibco™ Puromycin Dihydrochloride
Aminonucleoside antibiotic produced by Streptomyces alboniger
Supplier: Gibco™ A1113802
Description
Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.
- Used in cell biology as selection antibiotic in mammalian cell culture systems
- Recommended working concentration ranges from 0.2–5.0μg/mL
- Can be toxic to eukaryotic cells at concentrations as low as 1μg/mL
- Supplied at 10mg/mL in 20 mM HEPES buffer (pH 7.2–7.5)
Cell Culture, Cloning, Drosophila S2 Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation
Specifications
| Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
|
| 10 mg/mL | |
| Liquid | |
| Sterile-filtered | |
| Eukaryotic Selection⁄Stable Cell Line Generation | |
| 20 mL |
| Eukaryotic Cells, Prokaryotic Cells | |
| Mammalian Cell Culture, Insect Cell Culture | |
| Antibiotic | |
| HEPES | |
| Gibco™ | |
| Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.