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Gibco™ DMEM, powder, high glucose, pyruvate DFS Item

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

$51.00 - $96.25

Specifications

Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Form Powder
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
12800017 DFS Item
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Gibco™
12800017
10 x 1 L
Each for $96.25
 
12800082 DFS Item
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Gibco™
12800082
10 L
Each for $51.00
 
Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate
Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Powder
Standard Serum Supplementation
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Room Temperature
HeLa, 293, Cos-7, and PC-12
Animal Origin-free
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No HEPES, No Sodium Bicarbonate
Gibco™
SDS
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In vitro diagnostic