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Gibco™ Defined Trypsin Inhibitor
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Catalog No. R007100
Encompass
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100 mL
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R007100 100 mL
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Catalog No. R007100 Supplier Gibco™ Supplier No. R007100
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Description

Animal origin-free

Sterile, phosphate-buffered saline solution (1X) containing purified soybean trypsin inhibitor.

Sustainable packaging

Cell Culture, Corneal Epithelial Cell Culture, Keratinocyte Culture, Mammalian Cell Culture, Mammary Epithelial Cell Culture, Primary Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage minus 20 degrees C
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1 X
Product Type Trypsin inhibitor
Sterility Sterile-filtered
pH 7.4
Product Line Cascade Biologics
Quantity 100 mL
Shipping Condition Room Temperature
Source Animal origin-free
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Frequently Asked Questions (FAQs)

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.
Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.
Which cell lines can I culture in Diploid Growth Serum-Reduced Medium (SRM)?

Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.
How long can I use Diploid Growth Serum-Reduced Medium (SRM) supplemented with Diploid Growth Supplement?

Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.
Can I freeze or thaw my cells in Diploid Growth Serum-Reduced Medium (SRM)?

Most diploid cell lines can be frozen and thawed in Diploid Growth Serum-Reduced Medium. Consult the Diploid Growth Serum Reduced Medium User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019015_DiploidGrowthSerumReducedMedium_UG.pdf) for additional information.
How do I adapt my cells to Diploid Growth Serum-Reduced Medium (SRM)?

Cells growing in medium with serum can be transferred directly to Diploid Growth Serum-Reduced Medium without adaptation. Continue to monitor and passage cells for 2-3 passages until consistent growth is achieved. Cultures may benefit from supplementation with 1-2% serum.
Is Defined Trypsin Inhibitor a Kunitz-type inhibitor or Bowman-Birk inhibitor?

Defined Trypsin Inhibitor (Cat. No. R007100) is the larger trypsin inhibitor, therefore it is a Kunitz-type inhibitor
What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

For Research Use Only. Not for use in diagnostic procedures.