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Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

$323.00 - $1344.00

Specifications

Isolation Technology Silica Spin Column
Purification Time 15 min. (After sample homogenization)
Elution Volume 50–100 μL
Sample Type Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Final Product Type Total RNA
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Description

Description

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights
Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
Efficient—high yields of total RNA.
Fast—15 minutes after lysis step
Pure—an A260/280 ratio > 1.9
Convenient—spin columns are capped and assembled with collection tubes

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

Specifications

Specifications

Silica Spin Column
50–100 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Room Temperature
Up to 160 μg
15 min. (After sample homogenization)
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Not High-throughput Compatible (Manual)
Mini
Bacteria: ≤109 cells
Blood: 0.5 mL
Cells: ≤107
Tissue and Insects: 30 mg
Yeast: ≤4 x 108 cells
Enzymatic reactions: ≤100 μL
SDS
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For Research Use Only. Not for use in diagnostic procedures.