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Thermo Scientific™ GeneJET RNA Purification Kit
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
Supplier: Thermo Scientific™ K0731
Description
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
- Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
- Efficient—high yields of total RNA
- Fast—15 minutes after lysis step
- Pure—an A260/280 ratio > 1.9
- Convenient—spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
Chemical Identifiers
| Silica Spin Column | |
| 50–100 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Room Temperature | |
| Up to 160 μg |
| 15 min. (After sample homogenization) | |
| Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| Mini | |
| Bacteria: ≤109 cells, Blood: 0.5 mL, Cells: ≤107, Tissue and Insects: 30 mg, Yeast: ≤4 x 108 cells, Enzymatic reactions: ≤100 μL |
Specifications
| • 600 μL Proteinase K; store at room temperature until opened, -20°C after opening • 40 mL Lysis Buffer; room temperature • 40 mL Wash Buffer 1 (concentrated); room temperature • 23 mL Wash Buffer 2 (concentrated); room temperature • 30 mL Nuclease-free Water; room temperature • 50 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C. • 50 Collection Tubes (2 mL); room temperature • 50 Collection Tubes (1.5 mL); room temperature |
|
| 15 min. (After sample homogenization) | |
| Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| 50 Preps | |
| Room Temperature | |
| Up to 160 μg |
| Silica Spin Column | |
| 50–100 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Mini | |
| Bacteria: ≤109 cells, Blood: 0.5 mL, Cells: ≤107, Tissue and Insects: 30 mg, Yeast: ≤4 x 108 cells, Enzymatic reactions: ≤100 μL |
For Research Use Only. Not for use in diagnostic procedures.