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Invitrogen™ GeneBLAzer™ In Vivo Detection Kit

Catalog No. 12578134
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375 Reactions
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12578134 375 Reactions
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Catalog No. 12578134 Supplier Invitrogen™ Supplier No. 12578134
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Description

GeneBLAzer™ In Vivo Detection Kit

The GeneBLAzer™ In Vivo Detection Kit measures β-lactamase reporter activity in live mammalian cells, using the β-lactamase substrate CCF2-AM. The GeneBLAzer™ Detection System provides the following advantages:

  • Suitable for use as a sensitive reporter of gene expression in living mammalian cells using fluorescence microscopy.
  • Provides a ratiometric readout to minimize differences due to variability in cell number, substrate concentration, fluorescence intensity, and emission sensitivity
  • Uses a non-toxic substrate, allowing continued cell culturing after quantitative analysis.
  • Compatible with a wide variety of applications, including microplate-based transcriptional assays and flow cytometry.
  • Provides a flexible and simple assay development platform for gene expression in mammalian cells.

    • Specifications

    Product Line GeneBLAzer
    Content And Storage In vivo detection kits contain 50 μg of CCF2-AM substrate (enough for 375 96-well reactions). Substrate should be stored at -20°C, dessicated, and protected from light. All reagents are guaranteed stable 6 months when properly stored.
    Detection Method Fluorescence
    Product Type Detection Kit
    Quantity 375 Reactions
    Format Kit
    Key Functions Reporter Assays
    Reporter Gene BLA (Beta-Lactamase)
    Substrate Beta-Lactamase Substrate

    Frequently Asked Questions (FAQs)

    Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

    The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
    - LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
    - LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

    Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

    Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).

    For Research Use Only. Not for use in diagnostic procedures.