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GE Healthcare HiTrap™ Protein A HP Prepacked Columns

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. GE Healthcare Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

$343.48 - $669.60

Specifications

Binding Capacity 20mg IgG/mL medium
For Use With (Application) For purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Storage Requirements 4° to 30°C
View More Specs
Products
Catalog Number Mfr. No. Flow Rate Quantity Price Quantity    

45-000-049

 
ge healthcare
17-0402-01
<4mL/min. 5 x 1mL Pack of 5 Each for $669.60

45-000-050

 
ge healthcare
17-0402-03
<4mL/min. 2 x 1mL Pack of 2 Each for $343.48

45-000-051

 
ge healthcare
17-0403-01
<20mL/min. 1 x 5mL Each for $624.96
Description & Specifications

Specifications

Binding Capacity 20mg IgG/mL medium
For Use With (Application) For purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Storage Requirements 4° to 30°C

  • Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
  • For use with syringe, pump or chromatography system such as KTA design or FPLC System
  • Binds 20mg human IgG/mL medium
  • Stable over pH 3 to 9 for use and 2 to 10 for cleaning
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns

Media
  • Ligand: Native protein A
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 9

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.