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Invitrogen™ Gateway™ pENTR™ 4 Dual Selection Vector 
Gateway™ pENTR™ 4 Dual Selection Vector
Supplier: Invitrogen™ A10465
Description
Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone.
The Gateway™ entry vectors (Table 1) offer the following:
- attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
- Kozak consensus sequence for efficient translation initiation in eukaryotic systems
- Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
- rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
- pUC origin for high-copy replication and maintenance of the plasmid in E. coli
- Kanamycin resistance gene for selection in E. coli
- The ccdB/chloramphenicol fusion gene located between the two attL sites for
- o negative selection and
- o Chloramphenicol selection in E. coli
- Kanamycin resistance gene for selection in E. coli
Specifications
| Dual SelectionExpression Vector | |
| Chloramphenicol (CmR), Kanamycin (KanR) | |
| Untagged | |
| 10 μg | |
| pENTR |
| 10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0. Store at -20°C |
|
| No Cleavage Site | |
| Gateway | |
| pENTR |
For Research Use Only. Not for use in diagnostic procedures.